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plasmid expressing suppressor of cytokine signaling 3 (socs3) pcmv socs-3  (Addgene inc)


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    Addgene inc plasmid expressing suppressor of cytokine signaling 3 (socs3) pcmv socs-3
    Plasmid Expressing Suppressor Of Cytokine Signaling 3 (Socs3) Pcmv Socs 3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv-socs3/pcmv+socs3/pmc09198438-83-1-17
    Average 90 stars, based on 1 article reviews
    plasmid expressing suppressor of cytokine signaling 3 (socs3) pcmv socs-3 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: Mammalian Innate Resistance to Highly Pathogenic Avian Influenza H5N1 Virus Infection Is Mediated through Reduced Proinflammation and Infectious Virus Release
    Article Snippet: Statistical analysis of the data in and was performed by a two-sample t test using Minitab 16 software. fig ft0 fig mode=article f1 Fig 6 caption a4 SOCS3 overexpression in human macrophages reduced USSR H1N1-induced TNF-α production. (A) Human macrophages were transfected with a SOCS3 expression plasmid (pCMV-SOCS3 from Addgene) and a GFP control vector (at a ∼50% transfection efficiency ... fig ft0 fig mode=article f1 Fig 8 caption a4 Pig respiratory epithelial cells released less infectious virus than did the corresponding human cells infected with HPAI H5N1 (A/turkey/Turkey/1/05) and USSR H1N1 (A/USSR/77) viruses. (A) Real-time PCR on 24-h-infected (MOI of 1) culture supernatants ...

    Article Title: c-Myb coordinates survival and the expression of genes that are critical for the pre-BCR checkpoint
    Article Snippet: To generate pMIG-SOCS3, SOCS3 cDNA was isolated from pCMV-SOCS3 ( 43 ) and cloned into the EcoRI site of pMIG-R1. pCMV-SOCS3 was provided by Dr. Ronald Kahn (AddGene plasmid #11486).

    Clone Assay:

    Article Title: Mammalian Innate Resistance to Highly Pathogenic Avian Influenza H5N1 Virus Infection Is Mediated through Reduced Proinflammation and Infectious Virus Release
    Article Snippet: Statistical analysis of the data in and was performed by a two-sample t test using Minitab 16 software. fig ft0 fig mode=article f1 Fig 6 caption a4 SOCS3 overexpression in human macrophages reduced USSR H1N1-induced TNF-α production. (A) Human macrophages were transfected with a SOCS3 expression plasmid (pCMV-SOCS3 from Addgene) and a GFP control vector (at a ∼50% transfection efficiency ... fig ft0 fig mode=article f1 Fig 8 caption a4 Pig respiratory epithelial cells released less infectious virus than did the corresponding human cells infected with HPAI H5N1 (A/turkey/Turkey/1/05) and USSR H1N1 (A/USSR/77) viruses. (A) Real-time PCR on 24-h-infected (MOI of 1) culture supernatants ...

    Article Title: c-Myb coordinates survival and the expression of genes that are critical for the pre-BCR checkpoint
    Article Snippet: To generate pMIG-SOCS3, SOCS3 cDNA was isolated from pCMV-SOCS3 ( 43 ) and cloned into the EcoRI site of pMIG-R1. pCMV-SOCS3 was provided by Dr. Ronald Kahn (AddGene plasmid #11486).

    Plasmid Preparation:

    Article Title: Mammalian Innate Resistance to Highly Pathogenic Avian Influenza H5N1 Virus Infection Is Mediated through Reduced Proinflammation and Infectious Virus Release
    Article Snippet: Statistical analysis of the data in and was performed by a two-sample t test using Minitab 16 software. fig ft0 fig mode=article f1 Fig 6 caption a4 SOCS3 overexpression in human macrophages reduced USSR H1N1-induced TNF-α production. (A) Human macrophages were transfected with a SOCS3 expression plasmid (pCMV-SOCS3 from Addgene) and a GFP control vector (at a ∼50% transfection efficiency ... fig ft0 fig mode=article f1 Fig 8 caption a4 Pig respiratory epithelial cells released less infectious virus than did the corresponding human cells infected with HPAI H5N1 (A/turkey/Turkey/1/05) and USSR H1N1 (A/USSR/77) viruses. (A) Real-time PCR on 24-h-infected (MOI of 1) culture supernatants ...

    Article Title: c-Myb coordinates survival and the expression of genes that are critical for the pre-BCR checkpoint
    Article Snippet: To generate pMIG-SOCS3, SOCS3 cDNA was isolated from pCMV-SOCS3 ( 43 ) and cloned into the EcoRI site of pMIG-R1. pCMV-SOCS3 was provided by Dr. Ronald Kahn (AddGene plasmid #11486).

    Software:

    Article Title: Mammalian Innate Resistance to Highly Pathogenic Avian Influenza H5N1 Virus Infection Is Mediated through Reduced Proinflammation and Infectious Virus Release
    Article Snippet: Statistical analysis of the data in and was performed by a two-sample t test using Minitab 16 software. fig ft0 fig mode=article f1 Fig 6 caption a4 SOCS3 overexpression in human macrophages reduced USSR H1N1-induced TNF-α production. (A) Human macrophages were transfected with a SOCS3 expression plasmid (pCMV-SOCS3 from Addgene) and a GFP control vector (at a ∼50% transfection efficiency ... fig ft0 fig mode=article f1 Fig 8 caption a4 Pig respiratory epithelial cells released less infectious virus than did the corresponding human cells infected with HPAI H5N1 (A/turkey/Turkey/1/05) and USSR H1N1 (A/USSR/77) viruses. (A) Real-time PCR on 24-h-infected (MOI of 1) culture supernatants ...

    Article Title: c-Myb coordinates survival and the expression of genes that are critical for the pre-BCR checkpoint
    Article Snippet: To generate pMIG-SOCS3, SOCS3 cDNA was isolated from pCMV-SOCS3 ( 43 ) and cloned into the EcoRI site of pMIG-R1. pCMV-SOCS3 was provided by Dr. Ronald Kahn (AddGene plasmid #11486).

    Over Expression:

    Article Title: Mammalian Innate Resistance to Highly Pathogenic Avian Influenza H5N1 Virus Infection Is Mediated through Reduced Proinflammation and Infectious Virus Release
    Article Snippet: Statistical analysis of the data in and was performed by a two-sample t test using Minitab 16 software. fig ft0 fig mode=article f1 Fig 6 caption a4 SOCS3 overexpression in human macrophages reduced USSR H1N1-induced TNF-α production. (A) Human macrophages were transfected with a SOCS3 expression plasmid (pCMV-SOCS3 from Addgene) and a GFP control vector (at a ∼50% transfection efficiency ... fig ft0 fig mode=article f1 Fig 8 caption a4 Pig respiratory epithelial cells released less infectious virus than did the corresponding human cells infected with HPAI H5N1 (A/turkey/Turkey/1/05) and USSR H1N1 (A/USSR/77) viruses. (A) Real-time PCR on 24-h-infected (MOI of 1) culture supernatants ...

    Article Title: c-Myb coordinates survival and the expression of genes that are critical for the pre-BCR checkpoint
    Article Snippet: To generate pMIG-SOCS3, SOCS3 cDNA was isolated from pCMV-SOCS3 ( 43 ) and cloned into the EcoRI site of pMIG-R1. pCMV-SOCS3 was provided by Dr. Ronald Kahn (AddGene plasmid #11486).

    Transfection:

    Article Title: Mammalian Innate Resistance to Highly Pathogenic Avian Influenza H5N1 Virus Infection Is Mediated through Reduced Proinflammation and Infectious Virus Release
    Article Snippet: Statistical analysis of the data in and was performed by a two-sample t test using Minitab 16 software. fig ft0 fig mode=article f1 Fig 6 caption a4 SOCS3 overexpression in human macrophages reduced USSR H1N1-induced TNF-α production. (A) Human macrophages were transfected with a SOCS3 expression plasmid (pCMV-SOCS3 from Addgene) and a GFP control vector (at a ∼50% transfection efficiency ... fig ft0 fig mode=article f1 Fig 8 caption a4 Pig respiratory epithelial cells released less infectious virus than did the corresponding human cells infected with HPAI H5N1 (A/turkey/Turkey/1/05) and USSR H1N1 (A/USSR/77) viruses. (A) Real-time PCR on 24-h-infected (MOI of 1) culture supernatants ...

    Article Title: c-Myb coordinates survival and the expression of genes that are critical for the pre-BCR checkpoint
    Article Snippet: To generate pMIG-SOCS3, SOCS3 cDNA was isolated from pCMV-SOCS3 ( 43 ) and cloned into the EcoRI site of pMIG-R1. pCMV-SOCS3 was provided by Dr. Ronald Kahn (AddGene plasmid #11486).

    Expressing:

    Article Title: Mammalian Innate Resistance to Highly Pathogenic Avian Influenza H5N1 Virus Infection Is Mediated through Reduced Proinflammation and Infectious Virus Release
    Article Snippet: Statistical analysis of the data in and was performed by a two-sample t test using Minitab 16 software. fig ft0 fig mode=article f1 Fig 6 caption a4 SOCS3 overexpression in human macrophages reduced USSR H1N1-induced TNF-α production. (A) Human macrophages were transfected with a SOCS3 expression plasmid (pCMV-SOCS3 from Addgene) and a GFP control vector (at a ∼50% transfection efficiency ... fig ft0 fig mode=article f1 Fig 8 caption a4 Pig respiratory epithelial cells released less infectious virus than did the corresponding human cells infected with HPAI H5N1 (A/turkey/Turkey/1/05) and USSR H1N1 (A/USSR/77) viruses. (A) Real-time PCR on 24-h-infected (MOI of 1) culture supernatants ...

    Article Title: c-Myb coordinates survival and the expression of genes that are critical for the pre-BCR checkpoint
    Article Snippet: To generate pMIG-SOCS3, SOCS3 cDNA was isolated from pCMV-SOCS3 ( 43 ) and cloned into the EcoRI site of pMIG-R1. pCMV-SOCS3 was provided by Dr. Ronald Kahn (AddGene plasmid #11486).

    Control:

    Article Title: Mammalian Innate Resistance to Highly Pathogenic Avian Influenza H5N1 Virus Infection Is Mediated through Reduced Proinflammation and Infectious Virus Release
    Article Snippet: Statistical analysis of the data in and was performed by a two-sample t test using Minitab 16 software. fig ft0 fig mode=article f1 Fig 6 caption a4 SOCS3 overexpression in human macrophages reduced USSR H1N1-induced TNF-α production. (A) Human macrophages were transfected with a SOCS3 expression plasmid (pCMV-SOCS3 from Addgene) and a GFP control vector (at a ∼50% transfection efficiency ... fig ft0 fig mode=article f1 Fig 8 caption a4 Pig respiratory epithelial cells released less infectious virus than did the corresponding human cells infected with HPAI H5N1 (A/turkey/Turkey/1/05) and USSR H1N1 (A/USSR/77) viruses. (A) Real-time PCR on 24-h-infected (MOI of 1) culture supernatants ...

    Article Title: c-Myb coordinates survival and the expression of genes that are critical for the pre-BCR checkpoint
    Article Snippet: To generate pMIG-SOCS3, SOCS3 cDNA was isolated from pCMV-SOCS3 ( 43 ) and cloned into the EcoRI site of pMIG-R1. pCMV-SOCS3 was provided by Dr. Ronald Kahn (AddGene plasmid #11486).

    Virus:

    Article Title: Mammalian Innate Resistance to Highly Pathogenic Avian Influenza H5N1 Virus Infection Is Mediated through Reduced Proinflammation and Infectious Virus Release
    Article Snippet: Statistical analysis of the data in and was performed by a two-sample t test using Minitab 16 software. fig ft0 fig mode=article f1 Fig 6 caption a4 SOCS3 overexpression in human macrophages reduced USSR H1N1-induced TNF-α production. (A) Human macrophages were transfected with a SOCS3 expression plasmid (pCMV-SOCS3 from Addgene) and a GFP control vector (at a ∼50% transfection efficiency ... fig ft0 fig mode=article f1 Fig 8 caption a4 Pig respiratory epithelial cells released less infectious virus than did the corresponding human cells infected with HPAI H5N1 (A/turkey/Turkey/1/05) and USSR H1N1 (A/USSR/77) viruses. (A) Real-time PCR on 24-h-infected (MOI of 1) culture supernatants ...

    Article Title: c-Myb coordinates survival and the expression of genes that are critical for the pre-BCR checkpoint
    Article Snippet: To generate pMIG-SOCS3, SOCS3 cDNA was isolated from pCMV-SOCS3 ( 43 ) and cloned into the EcoRI site of pMIG-R1. pCMV-SOCS3 was provided by Dr. Ronald Kahn (AddGene plasmid #11486).

    Infection:

    Article Title: Mammalian Innate Resistance to Highly Pathogenic Avian Influenza H5N1 Virus Infection Is Mediated through Reduced Proinflammation and Infectious Virus Release
    Article Snippet: Statistical analysis of the data in and was performed by a two-sample t test using Minitab 16 software. fig ft0 fig mode=article f1 Fig 6 caption a4 SOCS3 overexpression in human macrophages reduced USSR H1N1-induced TNF-α production. (A) Human macrophages were transfected with a SOCS3 expression plasmid (pCMV-SOCS3 from Addgene) and a GFP control vector (at a ∼50% transfection efficiency ... fig ft0 fig mode=article f1 Fig 8 caption a4 Pig respiratory epithelial cells released less infectious virus than did the corresponding human cells infected with HPAI H5N1 (A/turkey/Turkey/1/05) and USSR H1N1 (A/USSR/77) viruses. (A) Real-time PCR on 24-h-infected (MOI of 1) culture supernatants ...

    Article Title: c-Myb coordinates survival and the expression of genes that are critical for the pre-BCR checkpoint
    Article Snippet: To generate pMIG-SOCS3, SOCS3 cDNA was isolated from pCMV-SOCS3 ( 43 ) and cloned into the EcoRI site of pMIG-R1. pCMV-SOCS3 was provided by Dr. Ronald Kahn (AddGene plasmid #11486).

    Real-time Polymerase Chain Reaction:

    Article Title: Mammalian Innate Resistance to Highly Pathogenic Avian Influenza H5N1 Virus Infection Is Mediated through Reduced Proinflammation and Infectious Virus Release
    Article Snippet: Statistical analysis of the data in and was performed by a two-sample t test using Minitab 16 software. fig ft0 fig mode=article f1 Fig 6 caption a4 SOCS3 overexpression in human macrophages reduced USSR H1N1-induced TNF-α production. (A) Human macrophages were transfected with a SOCS3 expression plasmid (pCMV-SOCS3 from Addgene) and a GFP control vector (at a ∼50% transfection efficiency ... fig ft0 fig mode=article f1 Fig 8 caption a4 Pig respiratory epithelial cells released less infectious virus than did the corresponding human cells infected with HPAI H5N1 (A/turkey/Turkey/1/05) and USSR H1N1 (A/USSR/77) viruses. (A) Real-time PCR on 24-h-infected (MOI of 1) culture supernatants ...

    Article Title: c-Myb coordinates survival and the expression of genes that are critical for the pre-BCR checkpoint
    Article Snippet: To generate pMIG-SOCS3, SOCS3 cDNA was isolated from pCMV-SOCS3 ( 43 ) and cloned into the EcoRI site of pMIG-R1. pCMV-SOCS3 was provided by Dr. Ronald Kahn (AddGene plasmid #11486).



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